2015 Fiscal Year Final Research Report
Induction of intestinal epithelial cells from CDX2 inducible human ES cells
| Project/Area Number |
26840083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 幹細胞 / 腸管発生 / 転写因子 / CDX2 / NEUROG3 / ヒトES細胞 |
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| Outline of Final Research Achievements |
We have induced human CDX2 in human embryonic stem (hES) cells, and examined their marker expression by immnohistochemistry, revealing unexpected result that showed clear reduction of intestinal epithelial cells after CDX2 induction. Our parallel transcriptome analysis also showed high similarity to neural cell lineage, compared with another adult tissues. We expected NEUROG3 as a downstream gene for CDX2, because it is a marker gene for intestinal secretion cells, but NEUROG3 induced hES cells were differentiated into functional neural cells that show calcium flux and action potential with multiple data from electrophysiological experiments. Finally we could obtain these new observations with a logical validity, although we didn't expect them in our initial plan.
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| Free Research Field |
分子生物学
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