Phosphorylation of ICE1 and regulation of cold signaling by MAP kinase

2015 Fiscal Year Final Research Report

• Project/Area Number: 26840087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: University of Tsukuba
• Principal Investigator: MIURA Kenji 筑波大学, 生命環境系, 准教授 (00507949)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 低温シグナル伝達 / 低温ストレス応答 / キナーゼ / 植物 / サリチル酸

Outline of Final Research Achievements

ICE1, MYC transcription factor, plays an important role in cold signaling mechanism and in response to cold stress in plants. MPK4 encoding MAP kinase has been isolated as an interactor of ICE1. The mpk4 mutant exhibited cold sensitivity and several cold-inducible genes were down-regulated in the mutant. The gene expression profile at the time of exposure to cold stress was investigated in the mpk4 mutant by microarray analyses. When compared with published microarray data, expression profile of mpk4 was similar to that of mkk2 or ice1 mutant and was contrary to the CBF2 overexpression plants. These results suggested that cold signaling is regulated by MKK2-MPK4-ICE1-CBF pathway.

Free Research Field

植物分子・生理科学