Three dimensional mapping of Ciona neuropeptides based on the imaging mass spectrometry

2016 Fiscal Year Final Research Report

• Project/Area Number: 26840108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: Suntory Foundation for Life Sciences
• Principal Investigator: Osugi Tomohiro 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 研究員 (50507986)
• Research Collaborator: SHIRAISHI Akira 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 研究員 ; SATAKE Honoo 公益財団法人サントリー生命科学財団, 生物有機科学研究所・統合生体分子機能研究部, 主幹研究員 ; SUGAHARA Kohtaro 公益財団法人サントリー生命科学財団, 生物有機科学研究所・構造生命科学研究部, 研究員 ; YAMAGAKI Tohru 公益財団法人サントリー生命科学財団, 生物有機科学研究所・構造生命科学研究部, 主席研究員 ; SUEMATSU Makoto 慶応義塾大学, 医学部, 教授 ; KAGEYAMA Akira 神戸大学, 大学院システム情報学研究科, 教授 ; SAKAMOTO Naohisa 神戸大学, 大学院システム情報学研究科, 講師
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ホヤ / 神経ペプチド / 質量分析イメージング

Outline of Final Research Achievements

In this study, we analyzed the distribution of neuropeptides in the Ciona brain using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) imaging mass spectrometry (MS) based on our previous peptidomics data. Major Ciona neuropeptides were detected on a single brain section, and the images of their distributions were acquired with a spatial resolution of 20 micro meters. We further developed a method for high-resolution imaging MS by a combination of fluorescent microscope and imaging MS in the brain sections of transgenic Ciona that expresses fluorescent protein in neurons. The high-resolution imaging MS for the first time showed the distribution of four peptides in a single brain section at single-cell resolution. Both the high-resolution imaging MS and immunohistochemistry using specific antiserum against the neuropeptide Ci-TK-I yielded similar results, suggesting that the high-resolution imaging MS has a potential to serve as a new platform for histology.

Free Research Field

神経内分泌