DNA barcording analysis on oral mircorbiome in ancient dental calculus Japanese skeltal remain

2016 Fiscal Year Final Research Report

• Project/Area Number: 26840158
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical anthropology
• Research Institution: Asahi University
• Principal Investigator: Yano Wataru 朝日大学, 歯学部, 助教 (80600113)
• Research Collaborator: Amanda Henry G. マックスプランク進化人類学研究所 ; IMAI Hiroo (60314176) ; HAYAKAWA Takashi 京都大学, 霊長類研究所, 特定助教 (00758493)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 口腔細菌叢 / 次世代シーケンサー / DNAメタバーコーディング解析 / 縄文人 / 歯石 / 歯周病 / 霊長類

Outline of Final Research Achievements

In the planned research, I successfully analyzed oral microbiome of ancient human population of Japan (Jomon people) using DNA meta-barcording technique with Next generation sequencer (MiSeq, Illumina). I used ancient remain of dental calculus from Jomon people in Kyoto University. The material contained representative oral pathogenic species such as porphyromonas spp. which indicates the possible infection of periodontal diseases. In addition to the human data, to explore the diversity of pathogenic oral microbial species of closely related primates species such as Chimpanzees (Pan troglodytes) and Lesser slow loris (Nycticebus pygmaeus) and found new species of oral pathogenic species.

Free Research Field

自然人類学