2015 Fiscal Year Final Research Report
TALEN-based mutagenesis for target genes in barley
Project/Area Number |
26850004
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Science in genetics and breeding
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Research Institution | Okayama University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2016-03-31
|
Keywords | 植物分子育種 / オオムギ / 形質転換 / ゲノム編集 / TALEN |
Outline of Final Research Achievements |
Transcription activator-like effector nuclease (TALEN)-vectors targeting for the genes encoding chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR) which were both involved in anthocyanin biosynthesis were constructed and applied to barley transformation. Although transgenic barley plants were obtained, any mutation was detected in target genes of these plants. On the other hand, 4 promoters regulating callus-specific gene expression were isolated in barley. For expression analysis, these promoters were cloned into expression vector of the gene encoding enhanced green fluorescent protein (EGFP). The transient expression of EGFP in callus was observed by all the promoters. In addition, 2 of 4 promoters showed stable expression of EGFP in transgenic callus in barley.
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Free Research Field |
農学
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