Identification of the MdMYB110a binding motif and the endogenous factors in the coloration of type 2 red-fleshed apples

2016 Fiscal Year Final Research Report

• Project/Area Number: 26850015
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: Nagoya University
• Principal Investigator: OTAGAKI Shungo 名古屋大学, 生命農学研究科, 助教 (50597789)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 赤果肉リンゴ / MYB転写因子 / 植物ホルモン / エチレン / アブシシン酸

Outline of Final Research Achievements

In this study, I aimed to identify the MdMYB110a binding motif and to clarify the contribution of plant hormones in the coloration of type 2 red-fleshed apples. For the first research topic, I performed the ectopic expression of MdMYB110a protein for chromatin immunoprecipitation analysis and the reporter assay using the agroinfiltration system but failed to identify the binding motif. For the second research topic, I revealed that the coloration of type 2 red-fleshed apples is not induced by ethylene, which is known as a major plant hormone accumulating during the apple fruit maturation, but by ABA.

Free Research Field

園芸科学