Pii-dependent resistance, as a model to elucidate molecular mechanism of effector-triggered immunity and pathogen recognition.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26850029
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Iwate Biotechnology Research Center
• Principal Investigator: Fujisaki Koki 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (30626510)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ETI / Exo70 / Pii / イネ / いもち病菌 / Integrated domain

Outline of Final Research Achievements

Currently, it has been known that plant NLR type immune receptors have integrated domains (IDs) derived from other host proteins. Some reports showed that IDs bind to pathogen effectors and enable NLRs to recognize pathogen effectors, directly. Therefore, IDs are regarded as important information to understand host-pathogen interactions.
In this study, we found the ID of rice NLR, Pii, and showed that the Pii-ID did not contribute to direct binding to cognate fungal effector, AVR-Pii. On the other hand, Pii-ID had crucial roles in the interaction with OsExo70-F3, an AVR-Pii-interactor required for Pii-dependent resistance, and in the recognition of AVR-Pii. These results indicate that Pii-ID may function by detecting host accessory protein targeted by pathogen effectors. This finding is different from the known ID function as pathogen effector-interacting domain, and may expand speculated functions of NLR-ID.

Free Research Field

植物病理