2016 Fiscal Year Final Research Report
Characterization of the enhanced protocatechuate decarboxylase and bio-based muconic acid production
| Project/Area Number |
26850041
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | muconic acid / protocatechuic acid / decarboxylase / bio-based monomer / lignin |
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| Outline of Final Research Achievements |
In this work, the mechanism of protocatechuate decarboxylation was investigated to develop an efficient production of a platform chemical, cis,cis-muconic acid (ccMA). The expression of KpdB, annotated as a 4-hydroxybenzoate decarboxylase subunit B, with protocatechuate decarboxylase (Pdc) could enhance Pdc activity, and it was revealed that KpdB is a flavin prenyltransferase and produces prenylated flavin mononucleotide as a cofactor of Pdc. By using the enhanced Pdc activity, a new ccMA producing bacterial strain that can produce ccMA from not only lignin model compounds but also rice straw lignin-derived aromatics was developed.
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| Free Research Field |
応用微生物学
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