2016 Fiscal Year Final Research Report
Bacterial catabolic system for the prevention of dead-end product formation
| Project/Area Number |
26850046
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
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| Research Collaborator |
HIGUCHI Yudai 長岡技術科学大学, 工学研究科・生物統合工学専攻
TAKENAMI Hiroki 長岡技術科学大学, 工学研究科
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | β-アリールエーテル / リグニン / Sphingobium属細菌 / カルボキシラーゼ / アセトフェノン / バニリン酸 |
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| Outline of Final Research Achievements |
In Sphingobium sp. strain SYK-6, a model bacterium for the lignin catabolism, the beta-arylether dimer is converted to vanilloyl acetic acid via the reductive ether cleavage. The resultant vanilloyl acetic acid is then subjected a non-enzymatic decarboxylation to generate acetovanillone. Here, we determined the catabolic pathway of acetovanillone and also identified the genes responsible for the first step of acetvaillone catabolism. In SYK-6, acetovanillone is initially carboxylated to produce vanilloyl acetic acid by a multiple component enzyme, AcdABCDEF, and then further converted to vanillate via the formation of coenzyme A ester. These findings enable us to understand the overview of beta-arylether catabolic pathway for the first time, and are also valuable for the biological lignin valorization.
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| Free Research Field |
応用微生物学
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