Identification of a long-chain fatty acid transporter in Corynebacterium glutamicum

2016 Fiscal Year Final Research Report

• Project/Area Number: 26850047
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: TAKENO Seiki 信州大学, 学術研究院農学系, 准教授 (30422702)
• Research Collaborator: IKEDA Masato 信州大学, 学術研究院農学系, 教授 (00377649)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: fatty acid transporter / C. glutamicum

Outline of Final Research Achievements

We attempted to identify a long-chain fatty acid transporter using fatty acid-producing strains of Corynebacterium glutamicum. After selecting four candidate genes for the transporter, we conducted single and multiple disruptions of these genes in the fatty acid-producing strain. The resulting quadruple-deletion strain exhibited severely impaired growth under culture conditions that enable extracellular production of fatty acids. However, such impairment was not observed under the conditions that do not permit fatty acid production. Similar results were observed only for the single-deletion strain in which a certain gene was disrupted. From these results, this gene is regarded to be the prospect involved in fatty acid transport. At present, there remains the possibility that one or more of the other candidates are also involved in the transport.

Free Research Field

応用微生物学