2014 Fiscal Year Research-status Report
水平伝播型発光遺伝子(lux）のクオラムセンシングによる発現調節機構の解明
| Project/Area Number |
26850051
|
|---|
| Research Institution | University of Miyazaki |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | クオラムセンシング / Vibrio / lux 遺伝子 |
|---|
| Outline of Annual Research Achievements |
We obtained whole genome sequence data of two strains of Vibrio chagasii and two strains of Photobacterium aquimaris using next-generation sequencing method. Genome sequence data of the four strains was analyzed, and potential quorum sensing genes were identified. The genome sequence data was also used to predict evolutionary history of lux operon genes in the four strains. Based on the whole genome sequence data we also started analysis of lux operon genes expression in the four strains. Furthermore, we started construction of V. chagasii mutants with disruptions in quorum sensing genes. The mutants are being constructed for both V. chagasii strains.
|
| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The project is slightly delayed due to two problems we encountered. First, the quorum sensing system in two Photobacterium aquimaris strains is apparently very different from that in related Vibrionaceae species. We were only able to identify a single quorum sensing gene in the two strains. Second problem we encountered was with the lux operon of the two V. chagasii strains. Based on the genome sequence data we obtained both V. chagasii strains contain horizontally transferred lux operons. However, identification of the lux operon genes donor requires whole genome sequencing of additional V. chagasii strains.
|
| Strategy for Future Research Activity |
In this budget year we will concentrate on analysis of the two V. chagasii strains. We will perform whole genome sequencing of additional two V. chagasii strains, which will allow us to predict evolutionary history of lux operons in V. chagasii. Furthermore, we will construct mutant V. chagasii strains with disruptions in quorum sensing genes. Our focus will be on the homologs of luxR and luxO genes in the two V. chagasii strains. Analysis of expression of lux operon genes in the mutant strains will also be conducted.
|
| Causes of Carryover |
In the fiscal year 2014 we performed some experiments originally planned for the fiscal year 2015. As a results, "article costs" increased, and funds were moved from "travel expenses" and "miscellaneous" funds. Therefore, budget for the 2014 fiscal year has 54,010 yen left.
|
| Expenditure Plan for Carryover Budget |
54,010 yen remaining from the fiscal year 2014 will be used to purchase additional reagents for experiments planned in the fiscal year 2015.
|
