2015 Fiscal Year Final Research Report
Screening of carbohydrate phosphorylases enabling practical synthesis of oligosaccharides
| Project/Area Number |
26850062
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 酵素 / オリゴ糖 |
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| Outline of Final Research Achievements |
Phosphorylases are exolytic enzymes catalyzing phosphorolysis of particular glycosides to produce sugar 1-phosphate with strict substrate specificity. The reaction is reversible, enabling the practical synthesis of oligosaccharides. However, there is little variation among phosphorylases and this limits their utilization for the production of oligosaccharides. Therefore, it would be beneficial to identify phosphorylases with previously unreported substrate specificities. In this study, two phosphorylase homologues belonging to glycoside hydrolase family 130 are characterized to be novel phosphorylases showing distinct chain-length specificities toward β-1,2-oligomannan.
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| Free Research Field |
応用生物化学
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