Development of an absolute quantification method for food components in processed foods by 1H quantitative NMR

2015 Fiscal Year Final Research Report

• Project/Area Number: 26850091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: National Institute of Health Sciences
• Principal Investigator: Ohtsuki Takashi 国立医薬品食品衛生研究所, 食品添加物部, 主任研究官 (30401011)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: NMR / 定量 / 食品添加物

Outline of Final Research Achievements

In this study, we established and validated a method for determining the saccharin sodium content in various processed foods by solvent extraction and 1H-qNMR. In the recovery test, the proposed method gave satisfactory recoveries (87.2-103.4%) and repeatabilities (0.4-9.9%) for various processed foods. In the analysis of commercially processed foods containing saccharin sodium, all saccharin sodium contents determined by the proposed method were in good agreement with those obtained by a conventional method based on dialysis and HPLC. Moreover, this method can achieve rapid quantification and yields analytical data with traceability to the International System of Units (SI) without the need for an authentic analyte standard. Therefore, the proposed method is a useful and practical tool for the determination of saccharin sodium in processed foods.

Free Research Field

食品科学