2015 Fiscal Year Final Research Report
Elucidation of toxicity mechanism(s) of Pleurocybella porrigens and creation of enzymes with novel substrate cleavage properties.
| Project/Area Number |
26850110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Utsunomiya University (2015)
Shizuoka University (2014) |
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Principal Investigator |
Suzuki Tomohiro 宇都宮大学, バイオサイエンス教育研究センター, 准教授 (10649601)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | きのこ / スギヒラタケ |
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| Outline of Final Research Achievements |
We have tried to obtain the gene which is interacted with PPL by a yeast two-hybrid method. As a result, the mixture (rPPL and recombinant clone protein) showed protease activity. Occasionally PPL showed the protease activity when it contained a minor peak at m/z 13,000 in MALDI-TOF-MS. We isolated PP-13,000 by reversed phase liquid chromatography and partial amino acid sequence was determined by LC-MS/MS analysis.We constructed heterologous expression systems of recombinant PPL by the basidiomycete P. sordida YK-624.We developed genomic and transcriptomic database of this mushroom which was equipped with three search functions; Keyword Search, GO Tree View and BLAST Search.
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| Free Research Field |
木質科学
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