Transcriptome analysis to understand disease resistance traits in two Scomber species

2015 Fiscal Year Final Research Report

• Project/Area Number: 26850122
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Yazawa Ryosuke 東京海洋大学, その他部局等, 准教授 (70625863)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 耐病性 / サバ類養殖 / RNA-seq / トランスクリプトーム解析

Outline of Final Research Achievements

We found that chub mackerel (Scomber japonicas) shows higher mortality in the high water temperature periods comparison with closely related species, blue mackerel (S. australasicus). In the present study, we performed that transcriptome analysis to understand such a disease resistance traits showing in blue mackerel with NGS technology. As the results of RNA-seq and subsequent de novo assembly, 1,072 contigs for chub mackerel and 52,530 contigs for blue mackerel. We further conducted reciprocal best match to estimate orthologs between two species and 13,875 contigs were obtained. Using this orthologs list of two Scomber species, we found expression levels of several immune related genes were siginificantly different. Theses genes are mostly related to acute immune reaction and inflammation.

Free Research Field

魚類病態生理