Characterization of Cytokine Homologue Gene, Macrophage Migration Inhibitory Factor , in Kuruma Shrimp Marsupenaeus japonicus

2015 Fiscal Year Final Research Report

• Project/Area Number: 26850126
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Japan Fisheries Research and Education Agency
• Principal Investigator: INADA Mari 国立研究開発法人水産総合研究センター, 増養殖研究所, 任期付研究員 (50723558)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: クルマエビ / 生体防御因子 / サイトカイン / MIF / マクロファージ遊走阻止因子 / Vibrio penaeicida / WSSV/PRDV / RNA干渉

Outline of Final Research Achievements

Cytokines are known as signaling protein molecules for intercellular communication. The macrophage migration inhibitory factor (MIF) was discovered as a factor which controls the marmot macrophage migration in the culture supernatants of the marmot lymphocyte in vertebrates. In this study, we report the characterization of genes of MIF family such as MIF and D-dopachrome tautomerase (DDT; MIF-2) from kuruma shrimp, Marsupenaeus japonicus.The in silico analyses such as domain, homology and phylogenetic analyses were performed. In prediction of 3D structure, MIF family formed trimer.The gene expression of MjDDT increased at 6 hours after injection of heat-killed Vibrio penaeicida cells. These data suggested that MjDDT of MIF family may be important in innate immunity in kuruma shrimp.

Free Research Field

魚病