Elucidation of genetic change associated with selection of immune competence in swine

2015 Fiscal Year Final Research Report

• Project/Area Number: 26850173
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: Shinkai Hiroki 国立研究開発法人農業生物資源研究所, 動物生体防御研究ユニット, 任期付研究員 (30502687)
• Co-Investigator(Renkei-kenkyūsha): UENISHI Hirohide 国立研究開発法人農業生物資源研究所, 家畜ゲノム研究ユニット, 主任研究員 (80391556) ; KITANI Hiroshi 国立研究開発法人農業生物資源研究所, 動物生体防御研究ユニット, ユニット長 (30355739) ; TAKENOUCHI Takato 国立研究開発法人農業生物資源研究所, 動物生体防御研究ユニット, 主任研究員 (20292518) ; TAKAHAGI Yoichi 日本ハム株式会社, 中央研究所, 研究員 (80520379)
• Research Collaborator: SUZUKI Junichi インターファーム株式会社, 今金種豚農場 ; MATSUMOTO Toshimi 国立研究開発法人農業生物資源研究所, 農業生物先端ゲノム研究センター ; TOKI Daisuke 公益社団法人農林水産, 食品産業技術振興協会・農林水産先端技術研究所
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ブタ / 感染症 / 多型 / 抗病性 / 免疫 / 選抜 / 育種 / マイクロアレイ

Outline of Final Research Achievements

We had previously produced pigs with high immune competence by selection of phagocytic capacity, complement-activating capacity by alternative pathway, and antibody response after vaccination with swine erysipelas. In this study, we performed microarray analysis by using macrophages from pigs of high immune competence or control, and identified 44 immune genes showed more than three times difference in the changes of gene expression after ligand stimulation between pig groups. Furthermore, we searched polymorphisms which had biased distribution between the pig groups in 5 kb of promoter region of these genes, and estimated haplotypes consisting of these polymorphisms. As a result, we elucidated specific types of promoter which were considered to be significantly increased in frequency by selection of immune competence in the genes encoding RNASEL, SAMHD1, STAT3, TRIM21, and TMEM150C, respectively.

Free Research Field

分子免疫学