2015 Fiscal Year Final Research Report
Analysis of carotenoid biosynthesis regulatory mechanisms in lactic acid bacteria
| Project/Area Number |
26850176
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Animal production science
|
|---|
| Research Institution | National Agriculture and Food Research Organization |
|---|
Principal Investigator |
Hagi Tatsuro 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門 畜産物研究領域, 主任研究員 (00510257)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 乳酸菌 / カロテノイド / トランスクリプトーム / メタボローム / 酸化ストレス |
|---|
| Outline of Final Research Achievements |
To clarify the carotenoid-centered oxidative stress response mechanism in lactic acid bacteria (LAB), effects of aerobic culture condition on gene expressions and metabolites in carotenoid-producing Enterococcus gilvus was investigated using whole-transcriptome and metabolome analysis. It turned out that aerobic culture condition increased the level of gene expression of isoprenoid biosynthesis pathway, converting Acetyl-CoA to carotenoid via mevalonate, and metabolites related to Acetyl-CoA biosynthesis. These results indicate that isoprenoid biosynthesis pathway, which could be induced by aerobic condition, plays an important role in oxidative stress response mechanisms in LAB.
|
| Free Research Field |
農学
|
