2016 Fiscal Year Final Research Report
For discovery of new drugs; elucidation of correlation between reaction mechanisms and structural features in Ngb/Cgb
| Project/Area Number |
26860022
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ヘムタンパク質 / 分光学的測定 / 活性酸素種 / 一酸化窒素 |
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| Outline of Final Research Achievements |
Newly discovered 6-coordinated globins, neuroglobin (Ngb) and cytoglobin (Cgb), have been reported to protect tissues from various damages. In this work, we aimed to clarify the reaction mechanisms of Ngb and Cgb which can be novel targets for newly produced drugs. First, we have studied on several reactive oxygen and nitrogen species for Ngb and Cgb, and Cgb showed inhibition on a production of peroxynitrite. Since there was no scavenging activity for peroxynitrite in Cgb directly, we identified that Cgb could have an inhibitory effect on a production of peroxynitrite. Second, we also investigated on a correlation of enzymatic activities and structural features in Ngb. Although Ngb WT showed less scavenging activity for superoxide than 5-coordination mutant, we found that binding of a certain lipid increased a scavenging activity for superoxide with a slight formation of 5-coordination. As a consequence, we elucidated some parts of functional mechanisms in Ngb and Cgb.
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| Free Research Field |
物理系薬学
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