Development of sensitive and noninvasive diagnostic methods using chiral amino acid metabolomics in urine

2016 Fiscal Year Final Research Report

• Project/Area Number: 26860023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Kyushu University
• Principal Investigator: MIYOSHI YURIKA 九州大学, 薬学研究院, 特任助教 (80707175)
• Research Collaborator: HAMASE Kenji 九州大学, 大学院薬学研究院, 教授 (10284522) ; MITA Masashi 株式会社資生堂
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: アミノ酸 / メタボロミクス / 光学分割

Outline of Final Research Achievements

Highly sensitive and selective analytical systems for the simultaneous determination of D- and L-enantiomers of proteinogenic amino acids in urine were designed and developed. By using mass spectrometers as detectors of the system, target chiral amino acids could be detected without severe interference in the urine, and various D-amino acids were found in the urine of healthy volunteers and mice. Using the analytical systems developed in this research, chiral amino acid metabolomics in the urine of model mice of ischemic acute kidney injury could be performed, and it was suggested that 8 chiral amino acids such as D-asparagine, D-serine, D-alanine and D-proline in urine were the candidate biomarkers of renal dysfunction.

Free Research Field

分析化学