Development of glyco-typing methods for Rotavirus surveillance

2016 Fiscal Year Final Research Report

• Project/Area Number: 26860030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Osaka Ohtani University
• Principal Investigator: Yamada Keita 大阪大谷大学, 薬学部, 講師 (80584185)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ロタウイルス / 糖鎖アレイ / Ｎ-グリコリルノイラミン酸 / ハイマンノース型糖鎖 / 硫酸化糖鎖 / 複合型糖鎖

Outline of Final Research Achievements

In this study, we analyzed some rotaviruses-glycans interaction, and revealed that rotavirus binding glycans are different depending on the type of virus strain. We also analyzed N-glycans expressed on rotavirus particles, and found that glycan profiles of rotavirus is more complicated than the one reported in the past. These studies suggested that analysis of virus binding glycans and virus expressing glycans is useful for identifying rotavirus strain. In addition, we developed a device that can analyze virus binding glycans and virus expressing glycans simultaneously. I hope that this device will be applied to rotavirus surveillance.

Free Research Field

分析化学