2015 Fiscal Year Final Research Report
Revealing the mechanism of the tumor initiation and development due to the tumor immunosuppression by Prothymosin-alpha.
| Project/Area Number |
26860038
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | がん生物学 / 免疫学 |
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| Outline of Final Research Achievements |
How does cancer escape the host immune system? To reveal the molecular mechanism underlying tumor immunosuppression, I focused on the PTMA, one of the DAMPs, which may release the tumor microenvironment from injured malignant cells. To analyze the physiological interaction between tumor and immune cells, I first developed a novel 3D co-culture system where heterogenous immune cells could be communicated together during a long period. I also developed a novel non-radioactive cellular cytotoxicity assay to evaluate the tumor immunosuppression. In addition to such in vitro assays, I specified one in vivo physiological condition upon bacterial infection is closely similar with tumor microenvironment in terms of the expression of M2 macrophages, which is known to one of the immunosuppressive immune cells. They will be certainly useful tools to understand the uncertain cancer immunosurveillance.
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| Free Research Field |
免疫学
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