Regulation of cell growth and cell viability by polyamines at the level of translation depending on the structural changes of polyamine-RNA complexes

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Chiba Institute of Science
• Principal Investigator: TERUI YUSUKE 千葉科学大学, 薬学部, 准教授 (60433687)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: ポリアミン / 細胞増殖 / 細胞生存率 / 翻訳 / 酸化ストレス / CR配列 / 大腸菌 / 動物細胞

Outline of Final Research Achievements

Polyamines exist mostly as polyamine-RNA complexes in cells. It was found that polyamines enhanced the synthesis of specific proteins at the level of translation, which are important for cell growth and cell viability. A group of genes whose expression is enhanced by polyamines at the level of translation be referred to as a “polyamine modulon”.
In this study, we looked for new members of polyamine modulon under oxidative stress conditions in Eschrichia coli. It was found that synthesis of SoxR, EmrR, and GshA proteins was enhanced by polyamines at the level of translation. It was found that synthesis of eEF1A was enhanced by polyamines at the level of translation among various translation factors in mammalian cells. The CR sequence existed in eukaryotic mRNAs, which regulates protein synthetic activity and influences polyamine stimulation of protein synthesis.

Free Research Field

医歯薬学