2016 Fiscal Year Final Research Report
Study on drug discovery in the central nervous system targeting ERM protein
| Project/Area Number |
26860058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pharmacology in pharmacy
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ERMタンパク質 / ミクログリア / 炎症性サイトカイン |
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| Outline of Final Research Achievements |
Moesin (Msn), a member of Ezrin/Radixin/Moesin (ERM) proteins links between membrane proteins and actin cytoskeleton, and contributes to maintenance of cellular function and morphology. In this study, we examined the function of Msn in microglia. We found that Msn was involved in the release mechanism of tumor necrosis factor α in BV2 cells. In addition, Msn interacted with inducible nitric oxide synthase (iNOS) via ERM binding protein 50 (EBP 50). It was also revealed that the phagocytosis ability of BV2 cells knocked down by Msn was decreased due to the abnormal actin skeleton. Furthermore, activation of microglia by administration of LPS was attenuated in Msn knockout mice. These results suggest that it may be the foundation of drug discovery research for microglia targeting Msn.
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| Free Research Field |
神経薬理学
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