Individual seed epigenetics profiling for rice to detect unauthorized GM trait

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental and hygienic pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: Nakamura Kosuke 国立医薬品食品衛生研究所, 生化学部, 主任研究官 (60570926)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 作物 / 遺伝子組換え / DNA / 検知法 / メチル化

Outline of Final Research Achievements

Rice callus developed from one grain was cultured under various stress conditions during cellular differentiation. Then, DNA methylation patters in rice endogenous Actin1 promoter (Act1p) were analyzed in wild-type rice and genetically modified (GM) rice that has transgenic Act1p. The DNA methylation pattern of Act1p in the GM rice was found methylated as compared to that in the wild-type rice under low regeneration condition. Also, DNA methylation patterns of Cauliflower Mosaic Virus (CaMV) 35S RNA promoter (P35S) in transgenic construct inserted into GM crop genomes and crop-infected CaMV genomes were analyzed. Multivariable statistical analysis of DNA methylation patterns indicated that the GM crops were grouped different from the crop-infected CaMV. Detection of the DNA methylation patterns in P35S could differentiate the presence of the GM crops for food ingredients and/or the CaMV in food commodities.

Free Research Field

食品化学