The pathological role of an orphan transporter in the regulation of cancer cell characteristics

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860109
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: ITO SHINGO 熊本大学, その他の研究科, 助教 (20466535)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: オーファントランスポーター / がん / プロテオミクス

Outline of Final Research Achievements

Low SLC22A18 protein expression has been reported to be associated with a poor survival rate in several cancers. In this study, we investigated the expression and functional role of SLC22A18 in HepG2 hepatoblastoma cells. Western blotting confirmed the protein expression of SLC22A18 in HepG2 cells. The invasiveness of SLC22A18-knockdown HepG2 cells was increased, while proliferation and chemotaxis of SLC22A18-knockdown HepG2 cells were reduced. Quantitative whole proteomic analysis using SWATH technology demonstrates a significant change in the intracellular proteins involved in cell proliferation in the SLC22A18-knockdown HepG2 cells. These findings suggest that SLC22A18 plays a pivotal role in the regulation of hepatocarcinoma cancer cell growth characteristics, by altering the expression of cellular proteins.

Free Research Field

分子薬剤学