Elucidation of the 3D structure and morphological diversity of the Golgi apparatus by modern 3D imaging technique

2016 Fiscal Year Final Research Report

• Project/Area Number: 26860128
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Asahikawa Medical College (2015-2016); Niigata University (2014)
• Principal Investigator: Koga Daisuke 旭川医科大学, 医学部, 准教授 (30467071)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ゴルジ装置 / 相関顕微鏡観察法 / Array tomography / 3D再構築法

Outline of Final Research Achievements

The present study developed a novel three-dimensional (3D) imaging technique combining array tomography and osmium impregnation methods. This novel method was applied to analysis of the 3D shape of the entire Golgi apparatus, which exhibits a spatially complex structure. The results clearly demonstrate that complete Golgi apparatus structures are both diverse in shape and cell type-dependent, with each apparatus appearing as a single mass located within a large cytoplasmic region. In addition, morphological changes of the Golgi apparatus were analyzed in gonadotropes before and after castration by correlative light and scanning electron microscopy paired with a combination of immunofluorescence imaging and osmium maceration methods.

Free Research Field

解剖学