Molecular mechanisms and roles of mitochondrial dynamics in development and diseases of myelinated axons

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860129
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: University of Yamanashi
• Principal Investigator: OHNO Nobuhiko 山梨大学, 総合研究部, 准教授 (10432155)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: Mitochondria / Myelination / Demyelination / Electron microscopy / Conductive resin

Outline of Final Research Achievements

We demonstrated alterations of mitochondrial distribution and dynamics during development of mouse central nervous system. In addition, changes of mitochondrial dynamics were caused specifically in demyelinated segments in the mouse dysmyelination model. In the 3D ultrastructural analyses using scanning electron microscopy, improvement of tissue preparation procedures and development of conductive resins were explored to achieve patent application. The carbon based conductive resin could reduce charging and improve imaging in the 3D ultrastructural analyses. The obtained results were published as several papers and presented in conferences.

Free Research Field

解剖学