Investigation on the involvement of purinergic signaling in the mechanism of photoaging

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Kitasato University
• Principal Investigator: KAWANO AYUMI 北里大学, 薬学部, 助教 (30631634)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: UVA / ケラチノサイト / ATP / サイトカイン / 分化

Outline of Final Research Achievements

The initiation of inflammatory response and dyskeratosis by UVA irradiation has large impact on photoaging. In this study, I examined the involvement of ATP in the production of cytokines and abnormal differentiation of UVA-irradiated keratinocytes. The concentration of extracellular ATP was transiently increased after UVA irradiation. UVA induced the production of cytokines (IL-1α, IL-6 and IL-8), and changed the expression of differentiation markers, but these UVA-induced cellular responses were suppressed by pretreatment of apyrase and P2 antagonist. These results showed that ATP might be a key player in the onset of UVA-irradiation-induced photoaging.

Free Research Field

医歯薬学