2015 Fiscal Year Final Research Report
Elusiodation of novel spatiotemporal mechanisms of synapse elimination by groups of complement proteins
| Project/Area Number |
26860148
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Keio University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
Aricescu A. Radu University of Oxford, Welcome Trust Centre for Human Genetics Division of Structural Biology, Professor
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 補体 / シナプス |
|---|
| Outline of Final Research Achievements |
It has been suggested so far that classical complement pathway initiated by complement C1q is associated to synapse elimination, but spatio-temporal mechanism how to determine whether the synapse should be pruned or not is still unclear. This research focused not only on C1q but also on the downstream of classical complement signaling. Reconstitution of C1 complex by C1q, C1r and C1s showed the binding on neurons but the binding pattern was similar to the binding of C1q on neurons, suggesting that C1q was enough to bind on neurons. Interestingly, the labeling of C1q was well colocalized with the signals detected by antibody against specific pattern of sugar chains, and was abolished by the enzyme which degraded the sugar chains. As the other topic, in order to develop novel molecules to control synapse formation, artificial excitatory synapse connector was elaborately analyzed and synapse disconnectors were also designed and analyzed about the feature such as binding specificity.
|
| Free Research Field |
分子細胞生物学
|
