Understanding of circadian system in the central circadian clock using in vivo recording

2016 Fiscal Year Final Research Report

• Project/Area Number: 26860156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Nagoya University (2016); Hokkaido University (2014-2015)
• Principal Investigator: Ono Daisuke 名古屋大学, 環境医学研究所, 助教 (30634224)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 概日リズム / 視交叉上核 / 生後発達 / 発光イメージング / 蛍光イメージング

Outline of Final Research Achievements

The temporal order of physiology and behavior is regulated by the circadian pacemaker located in the suprachiasmatic nucleus (SCN). We identified VIP is essential for the tissue-level circadian rhythm in the neonatal SCN of CRY double deficient mice (Cry1,2-/-). On the other hand, AVP synthesis was significantly attenuated in the Cry1,2-/- SCN, which contributes to aperiodicity in the adult mice together with an attenuation of VIP signaling as a natural process of ontogeny (Ono et al., 2016 Science Advances).
We also established in vivo clock gene expression rhythms using an optical fiber, and found that The Per1 rhythm was phase-delayed instantaneously by the light in parallel with the activity-onset, whereas the Bmal1 rhythm was phase-delayed gradually similar to the activity-offset. Dissociation of Per1 and Bmal1 rhythms was also observed in the cultured SCN slice. These results suggest the existence of two oscillations with different molecular mechanisms (Ono et al., 2017 PNAS).

Free Research Field

概日リズム