2015 Fiscal Year Final Research Report
Pharmacogenetic analysis of orexin neuronal projections
| Project/Area Number |
26860157
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Jichi Medical University (2015)
Nagoya University (2014) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMANAKA Akihiro 名古屋大学, 環境医学研究所, 教授 (60323292)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | DREADD / オレキシン神経 / 視床下部 / 投射経路 |
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| Outline of Final Research Achievements |
Using orexin-Cre mice and Cre-dependent AAV vectors, we achieved pharmacogenetic activation of orexin neurons. We also achieved selective ablation of orexin neurons using diphtheria toxin fragment A (DTA). Our comprehensive analysis of these transgenic mice revealed a concurrent regulation of sleep/wakefulness, feeding, and metabolism by orexin neurons. In order to manipulate specific orexin neuronal projections, we developed genetic tools for retrograde transport of Cre recombinase.
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| Free Research Field |
神経生理学
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