2016 Fiscal Year Final Research Report
Elucidation of molecular mechanism for development of atherosclerosis by cytotoxic factors in cigarette smoke
| Project/Area Number |
26860166
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General pharmacology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
|
|---|
| Research Collaborator |
MAI Yosuke
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | タバコ煙ガス相 / 不飽和カルボニル化合物 / 細胞傷害 / プロテインキナーゼC |
|---|
| Outline of Final Research Achievements |
Cigarette smoking is one of the risk factors for cardiovascular diseases. The objective of this study is to elucidate the molecular mechanism(s) for cell injury induced by unsaturated carbonyl compounds in the gas phase of cigarette smoke. First, the method for preparation of gas phase extract of cigarette smoke was optimized. The cell injury caused by both gas phase extract of cigarette smoke and unsaturated carbonyl compounds was intracellular calcium-dependent. They also activated protein kinase C in intracellular calcium-dependent manner. Several antioxidants such as reduced form of glutathione and vitamin E suppressed cell injury induced by unsaturated carbonyl compounds.
|
| Free Research Field |
薬理学
|
