2016 Fiscal Year Final Research Report
The role of prostaglandin F2 alpha in the regulation of platelet function
| Project/Area Number |
26860167
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 血小板 / プロスタグランジンF2α / EP3受容体 / TP受容体 / 受容体欠損マウス |
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| Outline of Final Research Achievements |
In murine platelets, PGF2α potentiated adenosine diphosphate-induced platelet aggregation in a concentration-dependent manner, while PGF2α alone did not induce aggregation. In platelets lacking PGF2α receptor FP, however, PGF2α-induced potentiation was not significantly different from that in wild-type (WT) platelets. In contrast, the potentiation was significantly blunted in platelets lacking PGE2 receptor subtype EP3 or TXA2 receptor TP, and completely disappeared in platelets lacking both EP3 and TP. In vivo, intravenously administered PGF2α shortened the bleeding time in WT mice. Moreover, PGF2α increased mortality and thrombus formation in arachidonic acid-induced thromboembolism model.
In conclusion, PGF2α potentiates platelet aggregation via EP3 and TP, but not via FP, and the potentiating action may play important role under physiological and pathological conditions.
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| Free Research Field |
薬理学
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