2016 Fiscal Year Final Research Report
Genetic and streoscopic analyses for the pathogenic mechanisms of anorectal malformations
| Project/Area Number |
26860196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 総排泄腔 / 肛門直腸奇形 / Shh / 遺伝学的細胞標識 / 尿直腸中隔 / 3次元イメージング |
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| Outline of Final Research Achievements |
The division of the embryonic cloaca is the most essential event for the formation of digestive and urinary tracts. The defective development of the cloaca results in anorectal malformations (ARMs). The incidence of ARMs is approximately 1 in 5,000 human births; however, the underlying pathogenic mechanisms are still unclear. Although there have been several discussions on the development of the cloaca, stereoscopic analyses are rarely utilized. In the current study, I performed three-dimensional reconstruction analyses of early staged embryos. The time-course analysis visualized the division processes of the cloaca in both wildtype and genetically engineered mice. These results suggested the presence of possible remodeling of the epithelial cells during cloacal division. I believe that the current results provide the basis for understanding both cloacal development and the ARM pathogenesis.
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| Free Research Field |
発生医学
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