Analysis of pathogenesis using NGS in Merkel cell carcinoma associated polyomavirus

2016 Fiscal Year Annual Research Report

• Project/Area Number: 26860238
• Research Institution: Tottori University
• Principal Investigator: 松下 倫子 鳥取大学, 医学部, 助教 (70642198)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: JAK-STAT経路 / immunohistochemistry / NGS

Outline of Annual Research Achievements

メルケル細胞癌(MCC)は高齢者に多い予後不良な皮膚の神経原性悪性腫瘍である。本研究課題はMCPyVの感染が影響を及ぼしている病態規定因子を解明し、MCPyVとMCCの病態解明や治療法開発のために役立てることである。本年度は以下の成果を得た。

(1)MCPyV陽性と陰性のMCCでNGSを行ったところ、症例数が少なかったためかMCPyV有無での差は明瞭でなかった。(2)JAK-STAT経路をMCPyV陽性MCC、MCPyV陰性MCCで検討した結果、p-JAK2、p-STAT3の発現が多くの症例で確認された。

また、本年度はこれまでの成果であるAID（Activation-induced deaminase）、Hedgehog signal経路の研究成果を論文として作成し、投稿中である。

Research Products

• [Journal Article] Comment on 'Cytokeratin 20-negative Merkel cell carcinoma is infrequently associated with the Merkel cell polyomavirus'. (2016)
  • Author(s): Iwasaki T, Matsushita M, Nonaka D, Murakami I, Hayashi K.
  • Journal Title: Modern Pathology Volume: 29 Pages: 89-90
  • DOI: 10.1038/modpathol.2015.69.
  • Peer Reviewed
• [Journal Article] Lower expression of CADM1 and higher expression of MAL in Merkel cell carcinomas are associated with Merkel cell polyomavirus infection and better prognosis. (2016)
  • Author(s): Iwasaki T, Matsushita M, Nonaka D, Nagata K, Kato M, Kuwamoto S, Murakami I, Hayashi K.
  • Journal Title: Human Pathology Volume: 48 Pages: 1-8
  • DOI: 10.1016/j.humpath.2015.09.030.
  • Peer Reviewed
• [Presentation] Ecology of merkel cell polyomavirus in healthy skin among individuals in an asian cohort shows close agreement with interleukin-1 loop model in langerhans cell histiocytosis. (2016)
  • Author(s): Murakami I, Nakashima J, Hashida Y, Daibata M, Matsushita M, Iwasaki T, Kuwamoto S, Kato M, Nagata K, Hayashi K, Oka T, Yoshino T, Imamura T, Morimoto A, Imashuku S, Gogusev J, Jaubert F.
  • Organizer: 32nd Annual Meeting of the Histiocyte Society
  • Place of Presentation: Dublin (Ireland)
  • Year and Date: 2016-10-17 – 2016-10-19
  • Int'l Joint Research
• [Presentation] The relations of JAK-STAT signaling expressions to MCPyV-infection and outcome in Merkel cell carcinomas. (2016)
  • Author(s): Matsushita M, Iwasaki T, Nonaka D, Kuwamoto S, Nagata K, Kato M, Kitamura Y, Hayash K.
  • Organizer: XXXI International Congress of the International Academy of Pathology and 28th Congress of the European Society of Pathology
  • Place of Presentation: Cologne (Germany)
  • Year and Date: 2016-09-25 – 2016-09-29
  • Int'l Joint Research
• [Presentation] Expression of a long non-coding RNA NEAT1 is significantly higher in MCPyV-positive Merkel cell carcinomas than MCPyV-negative ones. (2016)
  • Author(s): Akizuki G, Imamura K, Yamada T, Mizutani R, Matsushita M, Iwasaki T, Nonaka D, Kuwamoto S, Nagata K, Hayashi K, Akimitsu N.
  • Organizer: XXXI International Congress of the International Academy of Pathology and 28th Congress of the European Society of Pathology
  • Place of Presentation: Cologne (Germany)
  • Year and Date: 2016-09-25 – 2016-09-29
  • Int'l Joint Research
• [Presentation] Biphasic production of immunoglobulin from Epstein-Barr virus reactivated lymphocytes in Graves' disease patients and controls. (2016)
  • Author(s): Nagata K, Kumata K, Nakayama Y, Satoh Y, Matsushita M, Kuwamoto S, Kato M, Sugihara H, Murakami I, Hayashi K.
  • Organizer: XXXI International Congress of the International Academy of Pathology and 28th Congress of the European Society of Pathology
  • Place of Presentation: Cologne (Germany)
  • Year and Date: 2016-09-25 – 2016-09-29
  • Int'l Joint Research
• [Presentation] メルケル細胞癌におけるJAK-STAT経路活性化とMCPyVおよび予後との関係 (2016)
  • Author(s): 松下倫子、岩崎健、野中大輔、桑本聡史、長田佳子、加藤雅子、北村幸郷、林一彦
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城）
  • Year and Date: 2016-05-12 – 2016-05-14
• [Presentation] メルケル細胞癌におけるHedgehog signal発現とMCPyV感染および予後との関連 (2016)
  • Author(s): 黒見晃行、松下倫子、岩崎健、野中大輔、桑本聡史、長田佳子、加藤雅子、北村幸郷、林一彦
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター（宮城）
  • Year and Date: 2016-05-12 – 2016-05-14