2015 Fiscal Year Final Research Report
The epigenetic analysis of benign and malignant biliary tract disease with crypt isolation technique
| Project/Area Number |
26860242
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Sugai Tamotsu 岩手医科大学, 病理学講座, 教授 (20187628)
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| Co-Investigator(Renkei-kenkyūsha) |
Sasaki Akira 岩手医科大学, 外科学講座, 教授 (40275540)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 胆管癌 / 癌抑制遺伝子 / DNAメチル化 |
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| Outline of Final Research Achievements |
We analysed the DNA methylation of its promoter region of cancer suppressor genes with cancerous glands and non-cancerous glands of bile duct cancer by crypt isolation technique. We verified the relation between the DNA methylation of cancer suppressor genes and bile duct cancer. In 31 cases of resected bile duct cancer, we picked up the fresh samples and collect cancerous glands and non-cancerous glands with crypt isolation technique. We extracted the DNA and analysed the DNA methylation of its promoter region by Bisulfite-pyrosequencing. We examined the frequencies of cancer related genes and the genome wide methylation.
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| Free Research Field |
人体病理学
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