2016 Fiscal Year Final Research Report
Identification of LEFTY as a molecular marker for ovarian clear cell carcinoma: its relationship to apoptosis and cell proliferation
| Project/Area Number |
26860245
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Research Collaborator |
SAEGUSA Makoto
KODERA Yoshio
KAWASHIMA Yusuke
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 卵巣明細胞癌 / ショットガンプロテオミクス / Lefty / バイオマーカー |
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| Outline of Final Research Achievements |
We focused on Lefty, the protein identified by shotgun proteomics analysis. Lefty expression level was significantly higher in OCCCas compared with non-OCCCas. OCCCa cells stably overexpressing Lefty reduced cell proliferation, along with decreased pSmad2 expression and either activated p53/p21waf1 pathway or increased p27kip1 expression directly or indirectly. Moreover, treatment of the stable cells with cisplatin exhibited increased apoptotic cells, together with inhibition of pSmad2-mediated XIAP expression and decreased ratio of bcl2/bax. In clinical samples, a significantly higher number of apoptotic cells and lower Ki-67 LI were observed in OCCCa with high Lefty score relative to those with low score. These findings suggest that Lefty may be an excellent OCCCa-specific molecular marker, which has anti-tumor effects altering cell proliferation and susceptibility to apoptosis.
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| Free Research Field |
人体病理学
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