Identification of cellular mRNAs targeted to mRNPs during infection

2017 Fiscal Year Final Research Report

• Project/Area Number: 26860256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Gunma University
• Principal Investigator: Seto Eri 群馬大学, 大学院医学系研究科, 助教 (40431382)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: P－body / mRNP / Trypanosoma cruzi

Outline of Final Research Achievements

P-bodies are cytoplasmic mRNP granules involved in translation and degradation of mRNAs. In this study, we investigated the effect of Trypanosoma cruzi（T. cruzi）infection on P-body assembly in host cells, and found that the number of P-body foci dramatically increased within 24h post-infection. RNAi-mediated knockdown of P-bodies significantly enhanced the infectivity and growth of T. cruzi. It was therefore expected that accumulated P-bodies may positively regulate the expression of mRNAs required for anti-parasitic immune responses. To identify cellular mRNAs targeted to P-bodies during infection, we purified P-bodies using immunoprecipitation, and analyzed the coimmunoprecipitated mRNAs by deep sequencing.

Free Research Field

微生物学