Genome-wide immunoscreening of antibodies responsible for Plasmodium falciparum growth inhibitory activity of Malian adult IgG

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860279
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: Morita Masayuki 愛媛大学, プロテオサイエンスセンター, 研究員 (60709632)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: マラリアワクチン候補抗原 / コムギ胚芽無細胞タンパク質合成法 / GIA / ハイスループットスクリーニング

Outline of Final Research Achievements

To identify novel malaria vaccine candidates, I used 1900 Plasmodium falciparum proteins prepared using wheat germ cell-free system. Using the proteins, I measured the immunoreactivity of Malian adult IgG samples. Antibody reactivities against 3 proteins were positively correlated with growth inhibitory activity against in vitro cultured P.falciparum. Immunoreactivity of LSA3 has the highest correlation coefficient. Anti LSA3 specific antibody purified from Malian adult IgGs showed growth inhibitory activity against P.falciparum. The results suggest that LSA3 is a blood-stage malaria vaccine candidate.

Free Research Field

寄生虫学