2016 Fiscal Year Final Research Report
Elucidation of the mechanism of outer membrane vesicles production in Gram-negative bacteria
| Project/Area Number |
26860293
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kyoto University (2015-2016)
University of Miyazaki (2014) |
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Principal Investigator |
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| Research Collaborator |
SHIRAISHI Tsukasa 札幌医科大学医学部, 微生物学講座, 助教
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 外膜小胞 / 国際共同研究 / HlyF |
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| Outline of Final Research Achievements |
The hemolysin F (hlyF) gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided with an enhanced production of outer membrane vesicles (OMVs) by bacteria expressing HlyF. The HlyF protein displays a predicted catalytic domain of the short chain dehydrogenase/reductase superfamily. This conserved domain was involved the ability of HlyF to promote the production of OMVs.
This study is the first evidence that pathogenic bacteria produce a virulence factor directly involved in the production of OMVs and expected that HlyF-dependent OMV production could be one of the method for the development of vaccine.
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| Free Research Field |
病原細菌
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