2016 Fiscal Year Final Research Report
Altered Exosomal miRNA expression of late onset acute graft-versus-host disease in allogeneic hematopoietic stem cell transplantation.
| Project/Area Number |
26860373
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Research Collaborator |
UMEZU Tomohiro 東京医科大学, 医学部, 講師 (40385547)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 移植片対宿主病 / 造血幹細胞移植 / エクソソーム / miRNA |
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| Outline of Final Research Achievements |
Exosomes, small endosome-derived extracellular vesicles, are present in various biological fluids, such as plasma, thus have potential in facilitating molecular diagnosis. The aim of this study is to determine clinically relevant exosomal miRNAs in patients developing LA GVHD after HSCT.
We evaluated blood samples obtained from 10 patients who underwent HSCT for treatment of hematologic diseases. Exosomal miRNA profiling was done using a TaqMan low-density array.
We found that a subset of miRNAs was significantly up-regulated in the exosomes of LA GVHD groups compared to non-GVHD controls. Accordingly, the predicted target genes of altered miRNAs were extracted by MirTarBase. To estimate the biological implication of LA GVHD related miRNAs, we then investigated comprehensively possible related pathway using DAVID bioinformatics tools. Our study indicates that altered expression of certain exosomal miRNAs may play an important role in the immunoregulation of LA GVHD.
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| Free Research Field |
血液学
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