Development of novel diagnosis of systemic lupus erythematosus by measurement of splicing activity

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860374
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Arito Mitsumi 聖マリアンナ医科大学, 医学部, 助教 (00509911)
• Co-Investigator(Renkei-kenkyūsha): NAGAI Kouhei 近畿大学, 生物理工学部, 講師 (70500578) ; SATO Toshiyuki 聖マリアンナ医科大学, 医学部, 助教 (10350430) ; KUROKAWA Manae 聖マリアンナ医科大学, 医学部, 大学院教授 (90301598) ; SUEMATSU Naoya 聖マリアンナ医科大学, 医学部, 准教授 (50202122) ; OKAMOTO Kazuki 聖マリアンナ医科大学, 医学部, 准教授 (40177085) ; KATO Tomohiro 聖マリアンナ医科大学, 医学部, 教授 (80233807)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 全身性エリテマトーデス / SLE / スプライシング

Outline of Final Research Achievements

It was studied whether‘measurement method of splicing activity of peripheral blood mononuclear cells (PBMCs)’is useful as a single diagnosis of systemic lupus erythematosus (SLE).
Firstly, the measurement method of splicing activity of PBMCs was developed and optimized. Next, the splicing activity of PBMCs from the patients with SLE and healthy donors was measeured by this methods. As a result of that, there was no difference on the splicing activity between groups of the patients with SLE and healthy donors. For a while, it was found that the measurement of splicing activity of only T cells may be more accurate than that of PBMCs, so I will investigate splicing activity of T cells from the patients with SLE and healthy donors.

Free Research Field

分子細胞生物学、分子病態学、疾患プロテオミクス