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2016 Fiscal Year Final Research Report

The effects of NKT cell-mediated immunotherapy by IDO inhibitor in mouse lung metastasis model

Research Project

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Project/Area Number 26860376
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Research InstitutionFujita Health University (2016)
Suzuka University of Medical Science (2014-2015)

Principal Investigator

Hoshi Masato  藤田保健衛生大学, 医療科学部, 講師 (40633996)

Research Collaborator SEISHIMA Mitsuru  
SAITO Kuniaki  
ITO Hiroyasu  
HARA Akira  
Project Period (FY) 2014-04-01 – 2017-03-31
Keywordsインドールアミン酸素添加酵素1 / インドールアミン酸素添加酵素2 / トリプトファン代謝産物 / B16F10細胞 / 抗腫瘍効果 / 細胞免疫療法
Outline of Final Research Achievements

The role of indoleamine 2,3-dioxygenase (Ido) in the L-tryptophan (Trp)-kynurenine (Kyn) pathway after lung metastasis model by injecting B16F10 cells was investigated. We used α-GalCer administrated mice of wild type (WT), IDO1 KO, IDO2 KO, and mice treated with 1-methyl-D or L-tryptophan (D or L-1MT), an inhibitor of Ido1 or Ido2 respectively, to study the importance of Trp-Kyn pathway metabolites.
The levels of Ido1 and Ido2 mRNA and protein expression in the lung from WT mice were significantly higher than those from non-injected WT mice. The anti-tumor effect in the lung from IDO1 KO mice or L-1MT treated mice was markedly improved compared to that in WT mice, IDO2 KO mice, and D-1MT treated mice. Moreover, the levels of Trp metabolites in lung from IDO1 KO mice or L-1MT treated mice was significantly decreased compared to that in lung from WT mice, IDO2 KO mice, and D-1MT treated mice.

Free Research Field

病態検査学

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Published: 2018-03-22  

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