2015 Fiscal Year Final Research Report
Development of efficient methods for preparation of high-affinity antibody mutants suitable for sensitive detection of small biomarker molecules
| Project/Area Number |
26860378
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Keywords | 抗体工学 / 臨床化学 |
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| Outline of Final Research Achievements |
Antibodies with high affinity and specificity work as a powerful tool for in vitro and in vivo clinical diagnosis. These antibodies have been prepared by the hybridoma method using immune spleen cells, but often lack sufficient binding abilities. Recent antibody engineering technology could improve the native antibodies, but considerable labor and period are required to select rare improved binders from a vast library of various mutated antibodies. To overcome this problem, this study aimed to develop innovative methods for isolating the improved species based on the flow cytometry principle. We prepared a key reagent for these systems, a monoclonal anti-phage antibody, and converted it to relevant single-chain Fv fragment (scFv). Moreover, this scFv was linked with a luminescent protein, and utility of this fusion protein was investigated.
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| Free Research Field |
医歯薬学
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