A method to differentiate peripheral neurons mediating itch from human induced pluripotent stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 26860386
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: Juntendo University
• Principal Investigator: UMEHARA Yoshie 順天堂大学, 医学(系)研究科(研究院), 博士研究員 (40707072)
• Research Collaborator: TOMINAGA Mitsutoshi 順天堂大学, 大学院医学研究科, 准教授 (50468592) ; MATSUDA Hironori 順天堂大学, 大学院医学研究科, 技術員
• Project Period (FY): 2014-04-01 – 2016-03-31
• Keywords: 末梢神経 / ヒトiPS細胞 / 難治性かゆみ

Outline of Final Research Achievements

We established a method to differentiate peripheral neurons from human induced pluripotent stem cells (hiPSCs). Our method provided robust induction of neural crest (NC) cells from hiPSCs. Moreover, human peripheral neurons were efficiently differentiated from hiPSCs-derived NC cells. This method enables detailed analysis of human DRG neurons and informs development of new therapies for patients with intractable pruritus such as atopic dermatitis.

Free Research Field

分子発生学、かゆみ科学