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2015 Fiscal Year Final Research Report

Direct detection of polioviruses from environmental samples

Research Project

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Project/Area Number 26860450
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Hygiene and public health
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Nakamura Tomofumi  国立感染症研究所, その他部局等, 研究員 (90446881)

Project Period (FY) 2014-04-01 – 2016-03-31
Keywordsポリオウイルス / 環境サーベイランス / レセプター結合型ビーズ / 次世代シークエンス
Outline of Final Research Achievements

BY using negatively-charged filter and magnetic nanobeads with PV receptor (PVR-MB), we could concentrate spiked PV to 5,000-fold conc. Besides this, entire capsid coding region amprification (ECRA) method and NGS analysis were highest sensitive at PV detection than any other methods. From the analysis of PV-positive Pakistani environmental samples, we could identify many kinds of PVs, including vaccine and wild strains, and other non-PV enteroviruses. It was stongly suggested that this combination method was high sensitive and time-saving PV detection tool with informative virus sequences. And this method is effective environmental PV surveillance technique without bias often found in cell culture based PV isolation.

Free Research Field

ウイルス学

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Published: 2017-05-10  

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