2016 Fiscal Year Final Research Report
Development of methods for body fluid identification based on DNA methylation.
| Project/Area Number |
26860472
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | National Research Institute of Police Science |
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Principal Investigator |
Watanabe Ken 科学警察研究所, 法科学第一部, 研究員 (20532047)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 物体検査 / 人体液斑の同定 / DNAメチル化 |
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| Outline of Final Research Achievements |
In this study, we developed MethyLight methods (quantitative real-time methylation-specific PCR) for quantitating the semen-specifically unmethylated region, DACT1 and the blood-specifically methylated region, cg06379435. The threshold methylation ratios for semen or blood identification were set by analyzing DNA from various body fluid samples using these methods. The use of these thresholds accurately identified semen or blood from other samples including highly aged semen and blood stains. Because a realtime PCR-based method requires no substantial work beyond preparing the PCR reaction mixture, simple and quick analysis may be realized with a reduced risk of contamination. Therefore, we suggest that these methods are more suited to forensic work.
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| Free Research Field |
法医生物学
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