2015 Fiscal Year Final Research Report
The regulation of duodenal epithelial barrier by the glial cell line-derived neurotrophic factor on chronic psychological stress
Project/Area Number |
26860484
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General internal medicine(including psychosomatic medicine)
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Research Institution | Osaka City University |
Principal Investigator |
TANAKA Fumio 大阪市立大学, 大学院医学研究科, 講師 (20623292)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Keywords | 心身医学 / ストレス |
Outline of Final Research Achievements |
The aim of this study is to elucidate the role of glial cell line-derived neurotrophic factor (GDNF) in duodenal epithelial barrier function. Mice were subjected to water avoidance stress as a chronic psychological stress and we investigated the duodenal protein expressions of GDNF and mRNA expressions of some kinds of cell-to-cell adhesion molecules. As a result, chronic stress suppressed the expression levels of GDNF in a time-dependent manner during the stress period. mRNA expression levels of E-cadherin, which was a kind of cell-to-cell adhesion molecules at the level of adherent junction, were significantly suppressed by the stress as well as GDNF. Other molecules did not have significant change. Furthermore, the expression levels of E-cadherin were positively correlated with the levels of GDNF. In conclusion, chronic stress down-regulated GDNF and E-cadherin, which were associated with duodenal epithelial barrier dysfunction at the level of adherent junction.
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Free Research Field |
内科(含心身医学)、消化器内科
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