2015 Fiscal Year Final Research Report
Epigenetic regulation of noncoding RNA in gastrointestinal stromal tumor
| Project/Area Number |
26860514
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Keywords | 消化管間質腫瘍 / エピジェネティクス / noncoding RNA |
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| Outline of Final Research Achievements |
We performed screening for detection of epigenetically silenced noncodingRNAs in GIST. For this purpose, GIST-T1 cells were treated by 5-aza-dc and 4-PBA. After which, expression of noncodingRNA and ChIP sequencing analysis were performed. We selected candidate genes which epigenetically silenced and found that miR-34a and miR-335 were frequently methylated in primary GIST. We also identified CpG island methylation of possible tumor suppressive lncRNA is associated with poor prognosis of GIST patients.
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| Free Research Field |
消化器がん
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